Figure 3: Effect of tanshinone IIA and salvianolic acid B on cell viability. After incubation with various concentrations of tanshinone IIA (1, 5, 10, 20, and 40 μM) and salvianolic acid B (5, 10, 20, 40, and 80 μM) for 48 h, cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. (a) No significant cytotoxicity was observed at tanshinone IIA concentrations lower than 10 μM. (b) Each test concentration of Salvianolic acid B showed no cytotoxicity in B16F10 melanoma cells. The result shown are expressed as a mean value ± standard error of the mean of three independent experiments and the data were analyzed by one-way analysis of variance followed by post hoc Tukey's test. (***P < 0.001 vs. control group).