Dermatologica Sinica

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 39  |  Issue : 1  |  Page : 33--40

Tanshinone IIA, a melanogenic ingredient basis of Salvia miltiorrhiza Bunge


Zhaojing Wang1, Huihao Tang1, Lili Yang2, Yiming Li1, Huali Wu1 
1 Department of TCM Chemistry, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
2 Department of Dermatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China

Correspondence Address:
Huali Wu
Department of TCM Chemistry, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai
China
Dr. Yiming Li
Department of TCM Chemistry, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai
China

Background: The roots of Salvia miltiorrhiza Bunge (Lamiaceae) have been often used to treat vitiligo in clinical for many years. However, the main ingredient basis of efficacy has not been known. Objectives: We investigated whether the two main constituents of S. miltiorrhiza, major hydrophobic compound tanshinone IIA (Tan IIA) and the major hydrophilic compound salvianolic acid B (Sal B), had the same melanogenic activity. Methods: To testify the potential roles of Tan IIA and Sal B in pigmentation, tyrosinase (Tyr) activity, melanin synthesis ability, and the molecular mechanisms stimulating melanin production were determined in B16F10 melanoma cells. Results: Tan IIA promoted melanogenesis and enhanced Tyr activity at its maximum concentration (10 μM), whereas that of Sal B had no effect. Furthermore, the color of cell pellets and morphological observation of B16F10 cells were visibly darkened. Regarding molecular mechanisms, Western blot results showed that Tan IIA (1, 3, and 10 μM) dose dependently increased the level of phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase MAPK, respectively. However, it decreased phosphorylation in extracellular signal-regulated protein kinase 1/2 MAPK signaling. Meanwhile, Tan IIA promoted the expression of microphthalmia-associated transcription factor and Tyr. Conclusion: Tan IIA might be a melanogenic ingredient basis of S. miltiorrhiza to increase the Tyr activity by activating the upstream MAPK signaling pathways, thereby contributing to pigmentary processing.


How to cite this article:
Wang Z, Tang H, Yang L, Li Y, Wu H. Tanshinone IIA, a melanogenic ingredient basis of Salvia miltiorrhiza Bunge.Dermatol Sin 2021;39:33-40


How to cite this URL:
Wang Z, Tang H, Yang L, Li Y, Wu H. Tanshinone IIA, a melanogenic ingredient basis of Salvia miltiorrhiza Bunge. Dermatol Sin [serial online] 2021 [cited 2021 Jul 25 ];39:33-40
Available from: https://www.dermsinica.org/article.asp?issn=1027-8117;year=2021;volume=39;issue=1;spage=33;epage=40;aulast=Wang;type=0