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  Indian J Med Microbiol
 

Figure 5: Shikonin promoted expression of apoptosis associated genes and proteins on HaCaT cell, which were suppressed by MiR-21 and EGF. (a) Relative expression of apoptosis associated mRNA. In the classical apoptotic signal pathway, both EGF and miR-21 inhibited the gene expression of caspase-3/-9 signal and promoted bcl-2 expression significantly. Shikonin suppressed miR-21-induced high expression of bcl-2, and antagonized EGF-induced low expression of caspase-3/-9. (b) Western blot analysis of the casepase-3/-9 apoptosis signal pathway in eight groups. Both EGF and miR-21 decreased the protein production of caspase-3, caspase-9 significantly, while shikonin increased their production and also reversed EGF-induced effect. This experiment repeated 3 times, all results were similar. * P < 0.05, ** P < 0.01 versus control group;o P < 0.05,## P < 0.01, “ P < 0.01. EGF: Epidermal growth factor

Figure 5: Shikonin promoted expression of apoptosis associated genes and proteins on HaCaT cell, which were suppressed by MiR-21 and EGF. (a) Relative expression of apoptosis associated mRNA. In the classical apoptotic signal pathway, both EGF and miR-21 inhibited the gene expression of caspase-3/-9 signal and promoted bcl-2 expression significantly. Shikonin suppressed miR-21-induced high expression of bcl-2, and antagonized EGF-induced low expression of caspase-3/-9. (b) Western blot analysis of the casepase-3/-9 apoptosis signal pathway in eight groups. Both EGF and miR-21 decreased the protein production of caspase-3, caspase-9 significantly, while shikonin increased their production and also reversed EGF-induced effect. This experiment repeated 3 times, all results were similar. * <i>P</i> < 0.05, ** <i>P</i> < 0.01 versus control group;<sup>o</sup> <i>P</i> < 0.05,<sup>##</sup> <i>P</i> < 0.01, “ <i>P</i> < 0.01. EGF: Epidermal growth factor