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  Indian J Med Microbiol
 

Figure 4: EGF and miR-21 promote proliferation of HaCaT cell, while inhibited by shikonin. (a) Relative expression of proliferation associated mRNA. In the NF-κB/IKKβ pathway, both EGF and miR-21 induced the two crucial gene expressions, and also promoted expression of PCNA. Shikonin antagonized their effect, especially reduced the high expression of IKKβ and PCNA. (b) Western blot analysis of the expressions of PCNA, NF-κB, and IKKβ in eight groups. Both EGF and miR-21 increased the protein production of PCNA significantly, and also invigorated NF-κB/IKKβ signals by promoting the generation of its key proteins (NF-κB, IKKβ). Shikonin decreased their expressions and also reversed miR-21-induced effect. This experiment repeated 3 times, all results were similar. * P < 0.05, ** P < 0.01 versus control group;o P < 0.05,## P < 0.01, “P < 0.01. EGF: Epidermal growth factor

Figure 4: EGF and miR-21 promote proliferation of HaCaT cell, while inhibited by shikonin. (a) Relative expression of proliferation associated mRNA. In the NF-κB/IKKβ pathway, both EGF and miR-21 induced the two crucial gene expressions, and also promoted expression of PCNA. Shikonin antagonized their effect, especially reduced the high expression of IKKβ and PCNA. (b) Western blot analysis of the expressions of PCNA, NF-κB, and IKKβ in eight groups. Both EGF and miR-21 increased the protein production of PCNA significantly, and also invigorated NF-κB/IKKβ signals by promoting the generation of its key proteins (NF-κB, IKKβ). Shikonin decreased their expressions and also reversed miR-21-induced effect. This experiment repeated 3 times, all results were similar. * <i>P</i> < 0.05, ** <i>P</i> < 0.01 versus control group;<sup>o</sup> <i>P</i> < 0.05,<sup>##</sup> <i>P</i> < 0.01, “<i>P</i> < 0.01. EGF: Epidermal growth factor