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  Indian J Med Microbiol
 

Figure 2: Gene expression of miR-21 was downregulated by Shikonin and miR-21 inhibitor. HaCaT cells were grown in either the presence or absence of EGF (10 ng/mL) for 24 h. The miR21 mimics and inhibitors were transfected for Lipofectamine 2000. (a1) miR-21 melting curve. (a2) miR-21 amplification curve. (b1) U6 melting curve. (b2) U6 amplification curve. The result of RT-PCR is valid, for the melting curve is unimodal, and peak position is annealing temperature. (c) Ratio of miR-21 mRNA expression in HaCaT cells of eight groups. ** P < 0.01 versus control group; P < 0.05,##P < 0.01, “ P < 0.01,o P < 0.05

Figure 2:  Gene expression of miR-21 was downregulated by Shikonin and miR-21 inhibitor. HaCaT cells were grown in either the presence or absence of EGF (10 ng/mL) for 24 h. The miR21 mimics and inhibitors were transfected for Lipofectamine 2000. (a1) miR-21 melting curve. (a2) miR-21 amplification curve. (b1) U6 melting curve. (b2) U6 amplification curve. The result of RT-PCR is valid, for the melting curve is unimodal, and peak position is annealing temperature. (c) Ratio of miR-21 mRNA expression in HaCaT cells of eight groups. ** <i>P</i> < 0.01 versus control group; <i>P</i> < 0.05,<sup>##</sup><i>P</i> < 0.01, “ <i>P</i> < 0.01,<sup>o</sup> <i>P</i> < 0.05