Dermatologica Sinica

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 37  |  Issue : 1  |  Page : 19--27

Differential expression of PTEN and PDCD4 tumor suppressors in melanoma and microRNA-21-positive melanoma cells and squamous carcinoma cells


Kao-Hui Liu1, Woan-Ruoh Lee2, Ya-Ju Hsieh3, Chia-Lun Chou4, Ming-Chung Jiang4, Shing-Chuan Shen5 
1 Department of Dermatology, Shuang-Ho Hospital, Taipei Medical University, New Taipei City; Department of Pharmacology, College of Medicine National Taiwan University, Taipei, Taiwan
2 Department of Dermatology, Shuang-Ho Hospital, New Taipei City; Graduate Institute of Medical Sciences; Department of Dermatology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
3 Department of Dermatology, Mackay Memorial Hospital, Hsinchu, Taiwan
4 Department of Dermatology, Shuang-Ho Hospital, New Taipei City; Department of Dermatology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
5 Graduate Institute of Medical Sciences; Department of Dermatology, School of Medicine; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan

Correspondence Address:
Shing-Chuan Shen
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, No. 250 Wu-Hsing Street, Hsing-Yi District, Taipei 11031
Taiwan

Background:In vitro cell experiments show that microRNA-21 downregulates the PTEN and PDCD4 tumor suppressor and promote melanoma cell proliferation and invasion. We examined microRNA-21, PTEN, and PDCD4 expressions in various melanoma cells as well as in melanoma specimens to define the actual expression profile of these tumor regulators. Materials and Methods: The microRNA-21, PTEN, and PDCD4 expressions in human keratinocytes and melanoma cells were analyzed by reverse-transcription polymerase chain reaction (RT-PCR) and real-time RT-PCR and immunoblotting. PTEN and PDCD4 expressions in melanoma patients were analyzed by immunohistochemistry. Results: RT-PCR and quantitative real-time PCR assays showed that A375 melanoma cells, squamous cell carcinoma (SCC-25), and SCC-4 skin squamous carcinoma cells expressed a higher level of microRNA-21 than HaCaT human keratinocytes. This inconsistent staining pattern of PTEN and PDCD4 in a melanoma tumor mass is not understandable, because the expression level of microRNA-21 in melanoma specimens are different. The expression of PDCD4 was not inversely correlated with the levels of microRNA-21 in these cells. In addition, we also found that only A2058 cells expressed low PTEN level and that A375, SCC-25, and SCC-4 cells expressed high PTEN levels. Furthermore, expression of PDCD4 was higher in the highly malignant B16F10 mouse melanoma cells than in B16 F0 cells; by contrast, both B16F0 and B16F10 cells expressed PTEN at high levels. Conclusion: Although PDCD4 and PTEN are targets of microRNA-21-dependent inhibition, PTEN and PDCD4 expressions are regulated in a more complex manner in skin cancer; not all microRNA-21-positive skin cancers certainly lose their normal PTEN and PDCD4 tumor suppressor functions.


How to cite this article:
Liu KH, Lee WR, Hsieh YJ, Chou CL, Jiang MC, Shen SC. Differential expression of PTEN and PDCD4 tumor suppressors in melanoma and microRNA-21-positive melanoma cells and squamous carcinoma cells.Dermatol Sin 2019;37:19-27


How to cite this URL:
Liu KH, Lee WR, Hsieh YJ, Chou CL, Jiang MC, Shen SC. Differential expression of PTEN and PDCD4 tumor suppressors in melanoma and microRNA-21-positive melanoma cells and squamous carcinoma cells. Dermatol Sin [serial online] 2019 [cited 2019 Sep 15 ];37:19-27
Available from: http://www.dermsinica.org/article.asp?issn=1027-8117;year=2019;volume=37;issue=1;spage=19;epage=27;aulast=Liu;type=0